Transketolase Monoclonal antibody, PBS Only

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  WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66016-1-Ig (same clone as 66016-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66016-1-Ig (same clone as 66016-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

Transketolase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66016-1-PBS

Clone No.3E2F3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

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Product Information

66016-1-PBS targets Transketolase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transketolase fusion protein Ag16779
• Full Name: transketolase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC009970
• Gene Symbol: Transketolase
• Gene ID (NCBI): 7086
• RRID: AB_11064729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29401
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.