Transketolase Monoclonal antibody, PBS Only

Transketolase Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E2F3

Cat no : 66016-1-PBS

Synonyms

TK, TKT, TKT1, Transketolase

---

Validation Data Gallery

• 

  WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66016-1-Ig (same clone as 66016-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66016-1-Ig (same clone as 66016-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66016-1-Ig(Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

---

Product Information

66016-1-PBS targets Transketolase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transketolase fusion protein Ag16779
• Full Name: transketolase
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC009970
• Gene Symbol: Transketolase
• Gene ID (NCBI): 7086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.