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Transketolase Monoclonal antibody

Transketolase Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E2F3

Cat no : 66016-1-Ig

Synonyms

TK, TKT, TKT1, Transketolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis using 66016-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of Jurkat using 66016-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human brain using 66016-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HeLa using 66016-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HepG2 using 66016-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human liver using 66016-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human liver using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human liver using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    IF Staining of HepG2 using 66016-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66016-1-Ig(Transketolase antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, human liver tissue, Jurkat cells, human brain tissue, HEK-293 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66016-1-Ig targets Transketolase in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Transketolase fusion protein Ag16779
Full Name transketolase
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC009970
Gene Symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

Protocols

Product Specific Protocols
WB protocol for Transketolase antibody 66016-1-IgDownload protocol
IHC protocol for Transketolase antibody 66016-1-IgDownload protocol
IF protocol for Transketolase antibody 66016-1-IgDownload protocol
FC protocol for Transketolase antibody 66016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Med

Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma

Authors - Yimin Sun
View Article
humanWB

FASEB J

Prolactin receptor potentiates chemotherapy through miRNAs-induced G6PD/TKT inhibition in pancreatic cancer

Authors - Ying-Na Liao
View Article