CoraLite® Plus 488-conjugated Transgelin 2 Monoclonal antibody

• Featured Product
• KD/KO Validated

Transgelin 2 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E5G1

Cat no : CL488-60044

Synonyms

TAGLN2, Transgelin 2/TAGLN2, SM22-alpha homolog, Epididymis tissue protein Li 7e, CDABP0035

---

Validation Data Gallery

• 

  IF Staining of human colon cancer using CL488-60044

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL488-60044

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Transgelin 2 antibody (CL488-60044, Clone: 2E5G1 ) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60044 targets Transgelin 2 in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transgelin 2 fusion protein Ag0337
• Full Name: transgelin 2
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC002616
• Gene Symbol: Transgelin-2/TAGLN2
• Gene ID (NCBI): 8407
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. Our test result showed that this antibody Is specific to TAGLN2. It does not bind TAGLN1 and TAGLN3.