Validation Data Gallery
|Positive IHC detected in||mouse heart tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue, HeLa cells, mouse heart tissue|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27867-1-AP targets Titin in IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Titin fusion protein Ag27496|
|Calculated molecular weight||3816 kDa|
|GenBank accession number||NM_001256850|
|Gene ID (NCBI)||7273|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.
Aberrant differentiation of second heart field mesoderm prefigures cellular defects in the outflow tract in response to loss of FGF8
Stem Cell Res
Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Víctor (Verified Customer) (01-26-2023)
The antibody is a bit dirty but specific for muscle cells. I'm trying to evaluate specific sarcomeric structures with higher magnifications, and they seem to be marked. So the antibody is OK.
Joseph (Verified Customer) (01-14-2020)
I tested the Titin antibody using IHC on 16uM mouse skeletal muscle sections (Tibialis anterior and Soleus) using 1:100 and 1:300 dilutions. Both dilutions worked very well.