CD141/Thrombomodulin Recombinant antibody, PBS Only

CD141/Thrombomodulin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240365D1

Cat no : 83416-4-PBS

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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Validation Data Gallery

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  WB analysis using 83416-4-RR (same clone as 83416-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83416-4-RR (Thrombomodulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung squamous cell carcinoma using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83416-4-RR (same clone as 83416-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83416-4-RR (same clone as 83416-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thrombomodulin antibody (83416-4-RR, Clone: 240365D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83416-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83416-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83416-4-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.15 nM.

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Product Information

The immunogen of 83416-4-PBS is CD141/Thrombomodulin Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: thrombomodulin
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 100-105 kDa
• GenBank Accession Number: NM_000361.3
• Gene Symbol: THBD
• Gene ID (NCBI): 7056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).