CoraLite® Plus 750-conjugated Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E12D10

Cat no : CL750-67992

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

---

Validation Data Gallery

• 

  FC experiment of HeLa using CL750-67992

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human Talin-1 (CL750-67992, Clone:2E12D10) (red), or 0.8 ug CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21) (CL750-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL750-67992 targets Talin-1 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Talin-1 fusion protein Ag5937
• Full Name: talin 1
• Calculated Molecular Weight: 2541 aa, 270 kDa
• Observed Molecular Weight: 270 kDa
• GenBank Accession Number: BC042923
• Gene Symbol: TLN1
• Gene ID (NCBI): 7094
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).