TYK2 Recombinant antibody

TYK2 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230176E7

Cat no : 83161-4-RR

Synonyms

JTK1, TYK2, tyrosine kinase 2

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Validation Data Gallery

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  WB analysis using 83161-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83161-4-RR (TYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 83161-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-4-RR, Clone: 230176E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of U2OS using 83161-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug TYK2 Recombinant antibody (83161-4-RR, Clone:230176E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83161-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83161-4-RR against Human TYK2 were performed. The affinity constant is 0.116 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: U2OS cells
• Positive FC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83161-4-RR is TYK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TYK2 fusion protein Ag28457
• Full Name: tyrosine kinase 2
• Calculated Molecular Weight: 1187 aa, 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC014243
• Gene Symbol: TYK2
• Gene ID (NCBI): 7297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.