TYK2 Recombinant antibody, PBS Only

TYK2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230176E7

Cat no : 83161-4-PBS

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2, 230176E7

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Validation Data Gallery

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  WB analysis using 83161-4-RR (same clone as 83161-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83161-4-RR (TYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83161-4-RR (same clone as 83161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83161-4-RR (TYK2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83161-4-RR (same clone as 83161-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-4-RR, Clone: 230176E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83161-4-RR (same clone as 83161-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug TYK2 Recombinant antibody (83161-4-RR, Clone:230176E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83161-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83161-4-RR against Human TYK2 were performed. The affinity constant is 0.116 nM.

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Product Information

The immunogen of 83161-4-PBS is TYK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TYK2 fusion protein Ag28457
• Full Name: tyrosine kinase 2
• Calculated Molecular Weight: 1187 aa, 134 kDa
• Observed Molecular Weight: 134 kDa
• GenBank Accession Number: BC014243
• Gene Symbol: TYK2
• Gene ID (NCBI): 7297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.