FcZero-rAb™ PE Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody

TWEAKR/CD266 FcZero-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

240991C5

Cat no : PE-FcA98125

Synonyms

CD266, TNFRSF12A, TweakR, FGF inducible 14, DR3LG

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Validation Data Gallery

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  FC experiment of HT-1080 using PE-FcA98125

  1x10^6 HT-1080 cells were surface stained with 5 ul PE Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody (PE-FcA98125, Clone: 240991C5) (red), or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: HT-1080 cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98125 targets TWEAKR/CD266 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TWEAKR/CD266 fusion protein Ag13823
• Full Name: tumor necrosis factor receptor superfamily, member 12A
• Calculated Molecular Weight: 129 aa, 14 kDa
• GenBank Accession Number: BC002718
• Gene Symbol: TWEAKR
• Gene ID (NCBI): 51330
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)