WB analysis using 28009-1-AP (same clone as 28009-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
WB analysis using 28009-1-AP (same clone as 28009-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28009-1-AP (same clone as 28009-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 28009-1-AP (same clone as 28009-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
WB analysis using 28009-1-AP (same clone as 28009-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human lung cancer using 28009-1-AP (same clone as 28009-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
TUBG2 Polyclonal antibody
Catalog Number
28009-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
