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TUBG2 Polyclonal antibody

TUBG2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28009-1-AP

Synonyms

tubulin, gamma 2, Tubulin gamma-2 chain, Tubulin gamma 2 chain, GTU-88, Gamma-2-tubulin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUBG2 Polyclonal antibody (28009-1-AP)

    WB analysis using 28009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TUBG2 Polyclonal antibody (28009-1-AP)

    WB analysis using 28009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TUBG2 Polyclonal antibody (28009-1-AP)

    IHC staining of human lung cancer using 28009-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TUBG2 Polyclonal antibody (28009-1-AP)

    IF Staining of HepG2 using 28009-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using TUBG2 Polyclonal antibody (28009-1-AP)

    FC experiment of HepG2 using 28009-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse cerebellum tissue, HeLa cells, T-47D cells, rat cerebellum tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28009-1-AP targets TUBG2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUBG2 fusion protein Ag27728
Full Name tubulin, gamma 2
Calculated Molecular Weight 451 aa, 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC051890
Gene Symbol TUBG2
Gene ID (NCBI) 27175
RRIDAB_2918138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TUBG2 antibody 28009-1-APDownload protocol
IHC protocol for TUBG2 antibody 28009-1-APDownload protocol
IF protocol for TUBG2 antibody 28009-1-APDownload protocol
FC protocol for TUBG2 antibody 28009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols