Filter: AllWBIHCIF/ICC

WB analysis using 66018-1-Ig (same clone as 66018-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 66018-1-Ig (same clone as 66018-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 66018-1-Ig (same clone as 66018-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 66018-1-Ig (same clone as 66018-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
WB analysis of rat liver using 66018-1-Ig (same clone as 66018-1-PBS)
rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
IHC staining of rat kidney using 66018-1-Ig (same clone as 66018-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66018-1-Ig (same clone as 66018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 66018-1-Ig (same clone as 66018-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

TST Monoclonal antibody, PBS Only

TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66018-1-PBS

Clone No.2A6C5

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Rhodanese, RDS, EC:2.8.1.1, 2A6C5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66018-1-PBS targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	TST fusion protein Ag9235 Predict reactive species
Full Name	thiosulfate sulfurtransferase (rhodanese)
Calculated Molecular Weight	297 aa, 33 kDa
Observed Molecular Weight	33 kDa
GenBank Accession Number	BC010148
Gene Symbol	TST
Gene ID (NCBI)	7263
RRID	AB_11045660
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16762
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.