TST Monoclonal antibody

TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

2A6C5

Cat no : 66018-1-Ig

Synonyms

Rhodanese, RDS, EC:2.8.1.1, 2A6C5

---

Validation Data Gallery

• 

  WB analysis of rat brain using 66018-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 66018-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66018-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66018-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66018-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66018-1-Ig (TST Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66018-1-Ig (TST Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 66018-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 66018-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: rat brain tissue, human brain tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below

Product Information

66018-1-Ig targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TST fusion protein Ag9235
• Full Name: thiosulfate sulfurtransferase (rhodanese)
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC010148
• Gene Symbol: TST
• Gene ID (NCBI): 7263
• RRID: AB_11045660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publications

Species	Application	Title
human, mouse	WB	J Biol Chem; Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfurtransfer kinetics.; Authors - Marouane Libiad
human	WB	Biochem Pharmacol; Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells.; Authors - Ashley A Untereiner
human	IHC	J Biol Chem; Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells.; Authors - Marouane Libiad
human	WB	Biomolecules; Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines.; Authors - Halina Jurkowska
rat	WB	Mol Biol Rep; Curcumin reduces myocardial ischemia-reperfusion injury, by increasing endogenous H2S levels and further modulating m6A; Authors - Jiankun Cui
mouse	WB	Burns; Cystathionine-gamma-lyase deficient mice are protected against the development of multiorgan failure and exhibit reduced inflammatory response during burn.; Authors - Akbar Ahmad