TSSK4 Recombinant antibody

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  WB analysis using 83401-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83401-4-RR (TSSK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 83401-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83401-4-RR (TSSK4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 83401-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using TSSK4 antibody (83401-4-RR, Clone: 240373F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83401-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83401-4-RR against Human TSSK4 were performed. The affinity constant is 5.41 nM.

TSSK4 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83401-4-RR

Clone No.240373F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20

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Tested Applications

• Positive WB detected in: A549 cells, HT-29 cells, COLO 320 cells, mouse testis tissue, mouse colon tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83401-4-RR targets TSSK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TSSK4 fusion protein Ag16205
• Full Name: testis-specific serine kinase 4
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC111088
• Gene Symbol: TSSK4
• Gene ID (NCBI): 283629
• RRID: AB_3671052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6SA08
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway.