TSPAN13 Polyclonal antibody

TSPAN13 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30072-1-AP

Synonyms

Tspan-13, Transmembrane 4 superfamily member 13, TM4SF13, Tetraspanin-13, Tetraspan NET-6

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Validation Data Gallery

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  WB analysis of PC-3 using 30072-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 30072-1-AP (TSPAN13 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30072-1-AP targets TSPAN13 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN13 fusion protein Ag32345
• Full Name: tetraspanin 13
• Calculated Molecular Weight: 204 aa, 22 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC033863
• Gene Symbol: TSPAN13
• Gene ID (NCBI): 27075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TSPAN13 (also known as NET6 or TM4SF13) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. Overexpression of TSPAN13 has been reported in prostate cancer, suggesting that TSPAN13 may have an important role in the progression of prostate cancer (PMID: 19148481). The experimentally determined molecular mass of 28-35 kDa is larger than the calcular molecular weight of 22 kDa, which could be a result of post-translational modifications (PMID: 23648579).