Anti-Human TSLP Rabbit Recombinant Antibody

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  FC experiment of HUVEC using 98247-1-RR

  1x10^6 untreated or IL-4, TNF-α and BFA-treated HUVEC cells were intracellularly stained with 0.25 ug Anti-Human TSLP Rabbit RecAb (98247-1-RR, Clone: 241821B2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98247-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98247-1-RR against Human TSLP were performed. The affinity constant is 2.85 nM.

TSLP Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98247-1-RR

Clone No.241821B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Thymic stromal lymphopoietin, 241821B2

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Tested Applications

• Positive FC (Intra) detected in: IL-4, TNF-α and BFA treated HUVEC cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98247-1-RR targets TSLP in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: thymic stromal lymphopoietin
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_033035.5
• Gene Symbol: TSLP
• Gene ID (NCBI): 85480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q969D9-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

TSLP is a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain, it mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. Another isoform of this protein may act as an antimicrobial peptide in the oral cavity and on the skin (PMID: 24850429).