TSG101 Monoclonal antibody, PBS Only

TSG101 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B7G8

Cat no : 67381-1-PBS

Synonyms

Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23

---

Validation Data Gallery

• 

  WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TSG101 antibody (67381-1-Ig, Clone: 2B7G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

  1X10^6 HeLa cells were stained with 0.5 ug Anti-Human TSG101 (67381-1-Ig, Clone:2B7G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67381-1-PBS targets TSG101 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TSG101 fusion protein Ag28569
• Full Name: tumor susceptibility gene 101
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 43-46 kDa
• GenBank Accession Number: BC002487
• Gene Symbol: TSG101
• Gene ID (NCBI): 7251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.