TRPC4AP Monoclonal antibody, PBS Only

TRPC4AP Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C10B1

Cat no : 66138-1-PBS

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

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Validation Data Gallery

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  WB analysis of human brain using 66138-1-Ig (same clone as 66138-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66138-1-Ig (same clone as 66138-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66138-1-Ig (same clone as 66138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66138-1-Ig (same clone as 66138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66138-1-Ig (same clone as 66138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66138-1-Ig (same clone as 66138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66138-1-PBS in a different storage buffer formulation.

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Product Information

66138-1-PBS targets TRPC4AP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRPC4AP fusion protein Ag18772
• Full Name: transient receptor potential cation channel, subfamily C, member 4 associated protein
• Calculated Molecular Weight: 797 aa, 91 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC013144
• Gene Symbol: TRPC4AP
• Gene ID (NCBI): 26133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.