TROVE2 Monoclonal antibody, PBS Only

TROVE2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4E5

Cat no : 67149-1-PBS

Synonyms

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

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Validation Data Gallery

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  WB analysis of HeLa using 67149-1-Ig (same clone as 67149-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67149-1-Ig (same clone as 67149-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67149-1-Ig (same clone as 67149-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67149-1-Ig (same clone as 67149-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67149-1-Ig (same clone as 67149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67149-1-PBS in a different storage buffer formulation.

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Product Information

67149-1-PBS targets TROVE2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TROVE2 fusion protein Ag4379
• Full Name: TROVE domain family, member 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC036658
• Gene Symbol: TROVE2
• Gene ID (NCBI): 6738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.