TROVE2 Monoclonal antibody

TROVE2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E5

Cat no : 67149-1-Ig

Synonyms

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, Ro 60 kDa autoantigen, RO60, RoRNP, Sjoegren syndrome antigen A2, SS A, SSA2, TROVE domain family member 2, TROVE domain family, member 2, TROVE2

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Validation Data Gallery

• 

  WB analysis of HeLa using 67149-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67149-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67149-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67149-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67149-1-Ig (TROVE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67149-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67149-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67149-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67149-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67149-1-Ig (TROVE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells, HEK-293 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67149-1-Ig targets TROVE2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TROVE2 fusion protein Ag4379
• Full Name: TROVE domain family, member 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC036658
• Gene Symbol: TROVE2
• Gene ID (NCBI): 6738
• RRID: AB_2882447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TROVE2, also named as RO60 or RoRNP, is a 538 amino acid protein, which is localized in cytoplasmic mRNP granules containing untranslated mRNAs. TROVE2 as a RNA-binding protein that binds to misfolded non-coding RNAs, pre-5S rRNA, and several small cytoplasmic RNA molecules known as Y RNAs. TROVE2 may stabilize some of these RNAs and protect them from degradation (PubMed:18056422). TROVE2 binds to endogenous Alu retroelements which are induced by type I IFN and stimulate porinflammaotry cytokine secretion.