CoraLite® Plus 488-conjugated TRMT10C Polyclonal antibody

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  IF Staining of U2OS using CL488-29087

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 TRMT10C antibody (CL488-29087) at dilution of 1:200.

TRMT10C Polyclonal Antibody for IF/ICC

Cat No. CL488-29087

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

RG9MTD1, MRPP1

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Tested Applications

• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-29087 targets TRMT10C in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRMT10C fusion protein Ag30642
• Full Name: RNA (guanine-9-) methyltransferase domain containing 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_017819
• Gene Symbol: TRMT10C
• Gene ID (NCBI): 54931
• RRID: AB_3672836
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7L0Y3
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).