CoraLite® Plus 488-conjugated TRIP10 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRIP10 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-10798

Synonyms

Cdc42 interacting protein 4, CIP4, HSTP, Protein Felic, Salt tolerant protein, STOT, STP, TR interacting protein 10, TRIP 10, TRIP10

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Validation Data Gallery

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  IF Staining of HeLa using CL488-10798

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 TRIP10 antibody (CL488-10798) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-10798 targets TRIP10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRIP10 fusion protein Ag1251
• Full Name: thyroid hormone receptor interactor 10
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC013002
• Gene Symbol: TRIP10
• Gene ID (NCBI): 9322
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.