TRIM72 Monoclonal antibody, PBS Only

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2C11

Cat no : 67403-1-PBS

Synonyms

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

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Validation Data Gallery

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  WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67403-1-Ig (same clone as 67403-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

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Product Information

67403-1-PBS targets TRIM72 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM72 fusion protein Ag2560
• Full Name: tripartite motif-containing 72
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC033211
• Gene Symbol: TRIM72
• Gene ID (NCBI): 493829
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.