TRIM54 Recombinant antibody

TRIM54 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230391G5

Cat no : 83175-2-RR

Synonyms

RNF30, Muscle-specific RING finger protein 3, Muscle-specific RING finger protein, MuRF-3, MuRF3

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Validation Data Gallery

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  WB analysis using 83175-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83175-2-RR (TRIM54 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 83175-2-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using TRIM54 antibody (83175-2-RR, Clone: 230391G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of MCF-7 using 83175-2-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Trim54 Recombinant Antibody (83175-2-RR, Clone:230391G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83175-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83175-2-RR against Human TRIM54 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: human heart tissue, mouse heart tissue
• Positive IF/ICC detected in: C2C12 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83175-2-RR targets TRIM54 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM54 fusion protein Ag15229
• Full Name: tripartite motif-containing 54
• Calculated Molecular Weight: 358 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC141807
• Gene Symbol: TRIM54
• Gene ID (NCBI): 57159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM54 (Tripartite motif-containing protein 54) is also names as MURF, MURF3, RNF30. The expression of TRIM54 is required for skeletal myoblast differentiation and myotube fusion. It is expressed in heart and skeletal muscle specifically (PMID:11243782). It has two isoforms with the molecular weight of 40KDa and 45KDa. It can be dimerizated and hetero-dimerizated to form homooligomer and heterooligomer (PMID:15967462).