TRIM46 Recombinant antibody, PBS Only (Capture)

TRIM46 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240557E1

Cat no : 83614-2-PBS

Synonyms

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

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Validation Data Gallery

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  WB analysis using 83614-2-RR (same clone as 83614-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 83614-2-RR (same clone as 83614-2-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83614-2-RR (same clone as 83614-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00591-1

  Cytometric bead array standard curve of MP00591-1, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-4-PBS. Standard: Ag15290. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00591-2

  Cytometric bead array standard curve of MP00591-2, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-3-PBS. Standard: Ag15290. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83614-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

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Product Information

83614-2-PBS targets TRIM46 as part of a matched antibody pair:

MP00591-1: 83614-2-PBS capture and 83614-4-PBS detection (validated in Cytometric bead array)

MP00591-2: 83614-2-PBS capture and 83614-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM46 fusion protein Ag15290
• Full Name: tripartite motif-containing 46
• Calculated Molecular Weight: 759 aa, 83 kDa
• GenBank Accession Number: BC069416
• Gene Symbol: TRIM46
• Gene ID (NCBI): 80128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.