TRIM46 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83614-2-RR (same clone as 83614-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 83614-2-RR (same clone as 83614-2-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83614-2-RR (same clone as 83614-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83614-2-RR (same clone as 83614-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00591-1

  Cytometric bead array standard curve of MP00591-1, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-4-PBS. Standard: Ag15290. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00591-2

  Cytometric bead array standard curve of MP00591-2, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-3-PBS. Standard: Ag15290. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83614-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

View 3D epitope

TRIM46 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83614-2-PBS

Clone No.240557E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

Tripartite, fibronectin type-III and C-terminal SPRY motif protein, Tripartite motif-containing protein 46, tripartite motif containing 46, TRIFIC, GeneY

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Product Information

83614-2-PBS targets TRIM46 as part of a matched antibody pair:

MP00591-1: 83614-2-PBS capture and 83614-4-PBS detection (validated in Cytometric bead array)

MP00591-2: 83614-2-PBS capture and 83614-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TRIM46 fusion protein Ag15290
• Full Name: tripartite motif-containing 46
• Calculated Molecular Weight: 759 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC069416
• Gene Symbol: TRIM46
• Gene ID (NCBI): 80128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z4K8
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).