CoraLite®594-conjugated TRIM25 Monoclonal antibody

TRIM25 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H6G1

Cat no : CL594-67314

Synonyms

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

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Validation Data Gallery

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  IF Staining of HeLa using CL594-67314

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated TRIM25 antibody (CL594-67314, Clone: 1H6G1 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67314 targets TRIM25 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM25 fusion protein Ag29325
• Full Name: tripartite motif-containing 25
• Calculated Molecular Weight: 630 aa, 71 kDa
• Observed Molecular Weight: 68-71 kDa
• GenBank Accession Number: BC016924
• Gene Symbol: TRIM25
• Gene ID (NCBI): 7706
• RRID: AB_2920111
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).