TRIM21 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

TRIM21 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E8B9

Cat no : 67136-1-PBS

Synonyms

RNF 81, RING finger protein 81, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM21, 52 kDa ribonucleoprotein autoantigen Ro/SS-A

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Validation Data Gallery

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  WB analysis using 67136-1-Ig (same clone as 67136-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67136-1-Ig (same clone as 67136-1-PBS)

  WB result of TRIM21 antibody (67136-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM21 transfected HeLa cells. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis using 67136-1-Ig (same clone as 67136-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67136-1-Ig (same clone as 67136-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67136-1-Ig (same clone as 67136-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67136-1-Ig (same clone as 67136-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67136-1-Ig (same clone as 67136-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67136-1-Ig (TRIM21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67136-1-Ig (same clone as 67136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67136-1-Ig (same clone as 67136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67136-1-Ig (same clone as 67136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67136-1-Ig (same clone as 67136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67136-1-Ig (TRIM21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67136-1-Ig (same clone as 67136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM21 antibody (67136-1-Ig, Clone: 1E8B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67136-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50371-2

  Cytometric bead array standard curve of MP50371-2, TRIM21 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67136-1-PBS. Detection antibody: 67136-3-PBS. Standard:Ag28377. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP50371-4

  Cytometric bead array standard curve of MP50371-4, TRIM21 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67136-1-PBS. Detection antibody: 67136-5-PBS. Standard:Ag28377. Range: 0.781-100 ng/mL.

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Product Information

67136-1-PBS targets TRIM21 as part of a matched antibody pair:

MP50371-2: 67136-1-PBS capture and 67136-3-PBS detection (validated in Cytometric bead array)

MP50371-4: 67136-1-PBS capture and 67136-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRIM21 fusion protein Ag28377
• Full Name: tripartite motif-containing 21
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 42-54 kDa
• GenBank Accession Number: BC010861
• Gene Symbol: TRIM21
• Gene ID (NCBI): 6737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules. TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.