TRAP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TRAP1 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5D4

Cat no : 67693-1-PBS

Synonyms

HSP 75, HSP75, HSP90L, TNFR associated protein 1, TRAP 1, TRAP1

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Validation Data Gallery

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  WB analysis using 67693-1-Ig (same clone as 67693-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

  WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 67693-1-Ig (same clone as 67693-1-PBS)

  IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67693-1-Ig (same clone as 67693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67693-1-Ig (same clone as 67693-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67693-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67693-1-PBS targets TRAP1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAP1 fusion protein Ag0400
• Full Name: TNF receptor-associated protein 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC000406
• Gene Symbol: TRAP1
• Gene ID (NCBI): 10131
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).