TRAP1 Monoclonal antibody

• Featured Product
• KD/KO Validated

TRAP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1H5D4

Cat no : 67693-1-Ig

Synonyms

TNFR associated protein 1, HSPC5, HSP90L, HSP75, HSP 75

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Validation Data Gallery

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  WB analysis of HeLa using 67693-1-Ig

  WB result of TRAP1 antibody (67693-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAP1 transfected HeLa cells.

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  WB analysis using 67693-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67693-1-Ig (TRAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 67693-1-Ig

  IP result of anti-TRAP1 (IP:67693-1-Ig, 5ug; Detection:67693-1-Ig 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of mouse brain using 67693-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67693-1-Ig (TRAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67693-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HeLa using 67693-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAP1 antibody (67693-1-Ig, Clone: 1H5D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67693-1-Ig targets TRAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAP1 fusion protein Ag0400
• Full Name: TNF receptor-associated protein 1
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC000406
• Gene Symbol: TRAP1
• Gene ID (NCBI): 10131
• RRID: AB_2882886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF receptor-associated protein 1 (TRAP1, synonym: HSP75) is a member of the HSP90 family of molecular chaperones, and is expressed ubiquitously and located to mitochondria. A unique LxCxE motif in HSP75, but not in other HSP90 family members, appears to be important for binding to the simian virus 40 T-antigen-binding domain of hypophosphorylated Rb. Data also suggests that suppression of the expression of TRAP1 in mitochondria plays an important role in the induction of apoptosis caused via formation of reactive oxygen species (ROS).

Publications

Species	Application	Title
human	WB	Virol Sin; Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus; Authors - Bo Wang