TRAIL Recombinant antibody

• 

  WB analysis using 84280-1-RR

  Human milk samples were subjected to SDS PAGE followed by western blot with 84280-1-RR (TRAIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

TRAIL Recombinant Antibody for WB, ELISA

Cat No. 84280-1-RR

Clone No.241419C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: human milk

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84280-1-RR targets TRAIL in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• RRID: AB_3671826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50591
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.