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TRAIL Recombinant antibody, PBS Only (Detector)

TRAIL Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241419C12

Cat no : 84280-1-PBS

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAIL Recombinant antibody (84280-1-RR)

    WB analysis using 84280-1-RR (same clone as 84280-1-PBS)

    Human milk samples were subjected to SDS PAGE followed by western blot with 84280-1-RR (TRAIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84280-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-1-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

Product Information

84280-1-PBS targets TRAIL as part of a matched antibody pair:

MP01166-1: 84280-2-PBS capture and 84280-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated Molecular Weight 281 aa, 33 kDa
Observed Molecular Weight22-25 kDa
GenBank Accession NumberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.