TPT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TPT1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G6A10

Cat no : 66713-1-PBS

Synonyms

FLJ27337, Fortilin, Histamine releasing factor, HRF, p02, p23, TCTP, TPT1

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Validation Data Gallery

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  WB analysis using 66713-1-Ig (same clone as 66713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66713-1-Ig (same clone as 66713-1-PBS)

  WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66713-1-Ig (same clone as 66713-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66713-1-Ig (same clone as 66713-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66713-1-PBS targets TPT1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPT1 fusion protein Ag27651
• Full Name: tumor protein, translationally-controlled 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003352
• Gene Symbol: TPT1
• Gene ID (NCBI): 7178
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.