TPR Recombinant antibody

TPR Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83177-5-RR
Clone No.230413A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Nucleoprotein TPR, 230413A2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83177-5-RR targets TPR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name translocated promoter region (to activated MET oncogene)
Calculated Molecular Weight 267 kDa
Observed Molecular Weight~270 kDa
GenBank Accession NumberNM_003292
Gene Symbol TPR
Gene ID (NCBI) 7175
RRIDAB_3670870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12270
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).

Protocols

Product Specific Protocols
WB protocol for TPR antibody 83177-5-RRDownload protocol
IHC protocol for TPR antibody 83177-5-RRDownload protocol
IF protocol for TPR antibody 83177-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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