CoraLite® Plus 488-conjugated TPMT Monoclonal antibody

TPMT Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D4B3

Cat no : CL488-68251

Synonyms

Thiopurine S-methyltransferase, EC:2.1.1.67, 1D4B3

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-68251

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 TPMT antibody (CL488-68251, Clone: 1D4B3 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68251 targets TPMT in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPMT fusion protein Ag28693
• Full Name: thiopurine S-methyltransferase
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005339
• Gene Symbol: TPMT
• Gene ID (NCBI): 7172
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.