TPI1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TPI1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E7E5

Cat no : 67515-1-PBS

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

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Validation Data Gallery

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  WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

  WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67515-1-PBS targets TPI1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TPI1 fusion protein Ag16653
• Full Name: triosephosphate isomerase 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007086
• Gene Symbol: TPI1
• Gene ID (NCBI): 7167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).