TOX2 Recombinant antibody, PBS Only

TOX2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241645C11

Cat no : 84304-5-PBS

Synonyms

GCX-1, GCX1, C20orf100, 241645C11

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Validation Data Gallery

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  WB analysis using 84304-5-RR (same clone as 84304-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84304-5-RR (TOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 84304-5-RR (same clone as 84304-5-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 84304-5-RR (same clone as 84304-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84304-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84304-5-RR against Human TOX2 were performed. The affinity constant is 110 nM.

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Product Information

84304-5-PBS targets TOX2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOX2 fusion protein Ag14772
• Full Name: TOX high mobility group box family member 2
• Calculated Molecular Weight: 488 aa, 52 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007636
• Gene Symbol: TOX2
• Gene ID (NCBI): 84969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).