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TOX2 Recombinant antibody

TOX2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241645C11

Cat no : 84304-5-RR

Synonyms

GCX-1, GCX1, C20orf100, 241645C11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOX2 Recombinant antibody (84304-5-RR)

    WB analysis using 84304-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84304-5-RR (TOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A549 cells using TOX2 Recombinant antibody (84304-5-RR)

    FC experiment of A549 using 84304-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of MCF-7 cells using TOX2 Recombinant antibody (84304-5-RR)

    FC experiment of MCF-7 using 84304-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84304-5-RR

    Affinity and Kinetic Characterization of 84304-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84304-5-RR against Human TOX2 were performed. The affinity constant is 110 nM.

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, HEK-293 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive FC (Intra) detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84304-5-RR targets TOX2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOX2 fusion protein Ag14772
Full Name TOX high mobility group box family member 2
Calculated Molecular Weight 488 aa, 52 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007636
Gene Symbol TOX2
Gene ID (NCBI) 84969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Protocols

Product Specific Protocols
WB protocol for TOX2 antibody 84304-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols