TOR1B Recombinant antibody, PBS Only (Detector)

TOR1B Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241574A7

Cat no : 84267-3-PBS

Synonyms

Torsin family 1 member B, Torsin 1B, DQ1, 241574A7

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Validation Data Gallery

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  WB analysis using 84267-3-RR (same clone as 84267-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84267-3-RR (TOR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84267-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01175-1

  Cytometric bead array standard curve of MP01175-1, TOR1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84267-1-PBS. Detection antibody: 84267-3-PBS. Standard: Ag16641. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01175-2

  Cytometric bead array standard curve of MP01175-2, TOR1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84267-2-PBS. Detection antibody: 84267-3-PBS. Standard: Ag16641. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84267-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84267-3-RR against Human TOR1B were performed. The affinity constant is 14.5 nM.

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Product Information

84267-3-PBS targets TOR1B as part of a matched antibody pair:

MP01175-1: 84267-1-PBS capture and 84267-3-PBS detection (validated in Cytometric bead array)

MP01175-2: 84267-2-PBS capture and 84267-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOR1B fusion protein Ag16641
• Full Name: torsin family 1, member B (torsin B)
• Calculated Molecular Weight: 336 aa, 38 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC015578
• Gene Symbol: TOR1B
• Gene ID (NCBI): 27348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Torsin A is 1 of 4 predicted mammalian torsin ATPases associated with assorted cellular activities (AAA+) proteins (PMID: 20015956). TOR1B (torsin family 1 member B), also called TorsinB (TorB), is similar to TorsinA at the sequence level. TorsinA and TorsinB are both ubiquitously expressed in all cell types though TorsinA is more highly expressed in neurons. TorsinA and TorsinB may be somewhat functionally redundant, with TorsinA being more important in neuronal cells and TorsinB being more important in other tissues (PMID: 24275647). TOR1B serve as a molecular chaperone assisting in the proper folding of secreted and/or membrane proteins,and plays a role in non-neural cells nuclear envelope and endoplasmic reticulum integrity (PMID: 24275647; 23569223).