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TOR1B Recombinant antibody

TOR1B Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241574A7

Cat no : 84267-3-RR

Synonyms

Torsin family 1 member B, Torsin 1B, DQ1, 241574A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOR1B Recombinant antibody (84267-3-RR)

    WB analysis using 84267-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84267-3-RR (TOR1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84267-3-RR

    Affinity and Kinetic Characterization of 84267-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84267-3-RR against Human TOR1B were performed. The affinity constant is 14.5 nM.

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84267-3-RR is TOR1B Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TOR1B fusion protein Ag16641
Full Name torsin family 1, member B (torsin B)
Calculated Molecular Weight 336 aa, 38 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC015578
Gene Symbol TOR1B
Gene ID (NCBI) 27348
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Torsin A is 1 of 4 predicted mammalian torsin ATPases associated with assorted cellular activities (AAA+) proteins (PMID: 20015956). TOR1B (torsin family 1 member B), also called TorsinB (TorB), is similar to TorsinA at the sequence level. TorsinA and TorsinB are both ubiquitously expressed in all cell types though TorsinA is more highly expressed in neurons. TorsinA and TorsinB may be somewhat functionally redundant, with TorsinA being more important in neuronal cells and TorsinB being more important in other tissues (PMID: 24275647). TOR1B serve as a molecular chaperone assisting in the proper folding of secreted and/or membrane proteins,and plays a role in non-neural cells nuclear envelope and endoplasmic reticulum integrity (PMID: 24275647; 23569223).

Protocols

Product Specific Protocols
WB protocol for TOR1B antibody 84267-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols