CoraLite® Plus 488-conjugated TOP2A Polyclonal antibody

• Featured Product
• KD/KO Validated

TOP2A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24641

Synonyms

DNA topoisomerase 2 alpha, TOP2, TOP2A, TP2A

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Validation Data Gallery

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  IF Staining of A431 using CL488-24641

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 TOP2A antibody (CL488-24641) at dilution of 1:400.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-24641 targets TOP2A in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOP2A fusion protein Ag19551
• Full Name: topoisomerase (DNA) II alpha 170kDa
• Calculated Molecular Weight: 1531 aa, 174 kDa
• Observed Molecular Weight: 174 kDa
• GenBank Accession Number: BC140791
• Gene Symbol: TOP2A
• Gene ID (NCBI): 7153
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.