TOLLIP Recombinant antibody, PBS Only

TOLLIP Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242098C6

Cat no : 84711-6-PBS

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP, 242098C6

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Validation Data Gallery

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  WB analysis using 84711-6-RR (same clone as 84711-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84711-6-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84711-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84711-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84711-6-RR against Human TOLLIP were performed. The affinity constant is 1.32 nM.

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Product Information

84711-6-PBS targets TOLLIP in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOLLIP fusion protein Ag1864
• Full Name: toll interacting protein
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC012057
• Gene Symbol: TOLLIP
• Gene ID (NCBI): 54472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).