TOLLIP Recombinant antibody
TOLLIP Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
242098C6
Cat no : 84711-6-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, HepG2 cells, PC-3 cells, HCT 116 cells, K-562 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84711-6-RR targets TOLLIP in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | TOLLIP fusion protein Ag1864 |
Full Name | toll interacting protein |
Calculated Molecular Weight | 30 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC012057 |
Gene Symbol | TOLLIP |
Gene ID (NCBI) | 54472 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).
Protocols
Product Specific Protocols | |
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WB protocol for TOLLIP antibody 84711-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |