Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68170-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of HepG2 using 68170-1-Ig
WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 68170-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of Jurkat using 68170-1-Ig
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse colon tissue, rat colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
U2OS cells
Positive FC (Intra) detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC
IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68170-1-Ig targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68170-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of HepG2 using 68170-1-Ig
WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.
IHC Figures
IHC staining of mouse colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of U2OS using 68170-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of Jurkat using 68170-1-Ig
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
TOLLIP Monoclonal antibody
Catalog Number
68170-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:400-1:1600 IF/ICC : 1:400-1:1600 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
