TOB1 Recombinant antibody

TOB1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241043A5

Cat no : 83944-1-RR

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

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Validation Data Gallery

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  WB analysis using 83944-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83944-1-RR (TOB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83944-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-1-RR against Human TOB1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, MCF-7 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83944-1-RR is TOB1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOB1 fusion protein Ag6714
• Full Name: transducer of ERBB2, 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC031406
• Gene Symbol: TOB1
• Gene ID (NCBI): 10140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#83944-1-RR recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.