TOB1 Recombinant antibody, PBS Only (Capture)

TOB1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241043A5

Cat no : 83944-1-PBS

Synonyms

Transducer of erbB 2 1, TOB, Protein Tob1, PIG49, APRO6

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Validation Data Gallery

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  WB analysis using 83944-1-RR (same clone as 83944-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83944-1-RR (TOB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83944-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00930-2

  Cytometric bead array standard curve of MP00930-2, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-3-PBS. Standard: Ag6714. Range: 0.156-20 ng/mL.

• 

  Cytometric bead array standard curve of MP00930-3

  Cytometric bead array standard curve of MP00930-3, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-2-PBS. Standard: Ag6714. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 83944-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-1-RR against Human TOB1 were performed. The affinity constant is below 1 pM.

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Product Information

83944-1-PBS targets TOB1 as part of a matched antibody pair:

MP00930-2: 83944-1-PBS capture and 83944-3-PBS detection (validated in Cytometric bead array)

MP00930-3: 83944-1-PBS capture and 83944-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TOB1 fusion protein Ag6714
• Full Name: transducer of ERBB2, 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC031406
• Gene Symbol: TOB1
• Gene ID (NCBI): 10140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.The predicted MW of this protein is 38 kDa. Catalog#83944-1-RR recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.