TNIK Monoclonal antibody, PBS Only

TNIK Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E9H3

Cat no : 67948-1-PBS

Synonyms

TNIK, TRAF2 and NCK-interacting protein kinase, 1E9H3, EC:2.7.11.1, KIAA0551

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Validation Data Gallery

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  WB analysis of K-562 using 67948-1-Ig (same clone as 67948-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human peripheral blood platelets using 67948-1-Ig (same clone as 67948-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67948-1-Ig (TNIK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67948-1-Ig (same clone as 67948-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67948-1-Ig (TNIK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TNIK antibody (67948-1-Ig, Clone: 1E9H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 67948-1-Ig (same clone as 67948-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TNIK (67948-1-Ig, Clone:1E9H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67948-1-PBS in a different storage buffer formulation.

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Product Information

67948-1-PBS targets TNIK in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TNIK fusion protein Ag30710
• Full Name: TRAF2 and NCK interacting kinase
• Calculated Molecular Weight: 155 kDa
• GenBank Accession Number: BC055427
• Gene Symbol: TNIK
• Gene ID (NCBI): 23043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.