TMPO/LAP2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TMPO/LAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14651-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 14651-1-AP (same clone as 14651-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14651-1-AP (same clone as 14651-1-PBS)

  WB result of TMPO/LAP2 antibody (14651-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMPO/LAP2 transfected HeLa cells. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14651-1-AP (same clone as 14651-1-PBS)

  IP result of anti-TMPO/LAP2 (IP:14651-1-AP, 4ug; Detection:14651-1-AP 1:500) with HeLa cells lysate 1600ug. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 14651-1-AP (same clone as 14651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14651-1-AP (TMPO/LAP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14651-1-AP (same clone as 14651-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (14651-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa cells using 14651-1-AP (same clone as 14651-1-PBS)

  IF result of anti-TMPO (14651-1-AP) in Hela cell by Dr. Ralph Kehlenbach. This data was developed using the same antibody clone with 14651-1-PBS in a different storage buffer formulation.

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Product Information

14651-1-PBS targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMPO/LAP2 fusion protein Ag6247
• Full Name: thymopoietin
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 75 kDa, 51 kDa and 39 kDa
• GenBank Accession Number: BC053675
• Gene Symbol: TMPO/LAP2
• Gene ID (NCBI): 7112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).