Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98263-1-RR

  1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone:241807A5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98263-1-RR

  1x10^6 HEK-293T cells or TMIGD2/CD28H transfected HEK-293T cells were stained with 0.25 ug Anti-Human TMIGD2/CD28H Rabbit Recombinant Antibody (98263-1-RR, Clone: 241807A5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98263-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98263-1-RR against Human TMIGD2/CD28H were performed. The affinity constant is 171 nM.

TMIGD2/CD28H {ptg:Brand} Recombinant Antibody for FC

Cat No. 98263-1-RR

Clone No.241807A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98263-1-RR targets TMIGD2/CD28H in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: transmembrane and immunoglobulin domain containing 2
• Calculated Molecular Weight: 282 aa, 31 kDa
• GenBank Accession Number: BC015655
• Gene Symbol: TMIGD2
• Gene ID (NCBI): 126259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96BF3
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704).