TMIGD2/CD28H Recombinant antibody, PBS Only

• 

  WB analysis using 84727-5-RR (same clone as 84727-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84727-5-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 84727-5-RR (same clone as 84727-5-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84727-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84727-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

TMIGD2/CD28H {ptg:Brand} Recombinant Antibody for WB, Indirect ELISA

Cat No. 84727-5-PBS

Clone No.241807C12

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, Indirect ELISA

Synonyms

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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Product Information

84727-5-PBS targets TMIGD2/CD28H in WB, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: transmembrane and immunoglobulin domain containing 2
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015655
• Gene Symbol: TMIGD2
• Gene ID (NCBI): 126259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96BF3
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).