TMIGD2/CD28H Recombinant antibody

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  WB analysis using 84727-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 84727-5-RR

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84727-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

TMIGD2/CD28H {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84727-5-RR

Clone No.241807C12

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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Tested Applications

• Positive WB detected in: Jurkat cells, pig thymus tissue, MOLT-4 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84727-5-RR targets TMIGD2/CD28H in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: transmembrane and immunoglobulin domain containing 2
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC015655
• Gene Symbol: TMIGD2
• Gene ID (NCBI): 126259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96BF3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).